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paper emdb emd 21547 cryo em map
Paper Emdb Emd 21547 Cryo Em Map, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paper+emdb+emd+21547+cryo+em+map/Streptococcus+pyogenes+Rosenbach/pm32544384-282-10-42
Average 94 stars, based on 11 article reviews
Paper Emdb Emd 21547 Cryo Em Map, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/paper+emdb+emd+21547+cryo+em+map/Streptococcus+pyogenes+Rosenbach/pm32544384-282-10-42
Average 94 stars, based on 11 article reviews
paper emdb emd 21547 cryo em map - by Bioz Stars,
2026-10
94/100 stars
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Cryo-EM Sample Prep:Article Title: Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LAMP-1 Santa Cruz Cat# sc-20011; RRID: AB_626853 Alexa Fluor Plus 594 Donkey anti-Mouse IgG Invitrogen Cat# A32744; RRID: AB_2762826 Bacterial and Virus Strains E. coli Top10 Thermo Fisher Scientific Cat# C404010 E. coli BL21(DE3) Novagen Cat# 69387-1 E. coli DH5a Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins n-Dodecyl-b-D-Maltoside (DDM) Anatrace Cat# D310S glyco-diosgenin (GDN) Anatrace Cat# GDN101 anti-Flag M2 affinity gel MilliporeSigma Cat# A2220 Flag peptide GenScript Cat# RP10586-1 Ni-NTA Agarose QIAGEN Cat# 30250 Dulbecco’s modified eagle’s medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) GIBCO Cat# 10565042 Fetal bovine serum (FBS) GIBCO Cat# 10091148 LB medium Sigma Cat# L3522 Freestyle 293 medium Thermo Fisher Scientific Cat# 12338018 Filipin MilliporeSigma Cat# F9765 Kanamycin MilliporeSigma Cat# K1876 Triton X-100 MilliporeSigma Cat# X-100 Lipofectamine LTX with PLUS Invitrogen Cat# 15338100 ProLong Gold antifade reagent Invitrogen Cat# P10144 DMEM GIBCO Cat# 11995073 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat# 15140163 Foetal Bovine Serum (FBS) Scientifix Cat# FFBS-500 Mycoplasma Removal Agent Bio-Rad Cat# BUF035 Bovine Serum Albumin Bovogen Cat# BSAS-AU Superose 6 Increase 10/300 GL GE Healthcare Cat# 29-0915-96 Superdex 200 Increase 10/300 GL GE Healthcare Cat# 28-9909-44 Source 15Q GE Healthcare Cat# 17094702 Asolectin MilliporeSigma Cat# 11145 Bio-Beads SM-2 Resin Bio-Rad Cat# 1523920 Deposited Data Coordinates of NPC1 in digitonin at pH 8.0 Gong et al., 2016 PDB: 3JD8 Coordinates of NPC1-nanodisc This paper PDB: 6W5R Coordinates of NPC1-8.0 This paper PDB: 6W5S Coordinates of NPC1-5.5a This paper PDB: 6W5T Coordinates of NPC1-5.5b This paper PDB: 6W5U Coordinates of NPC1-NPC2 complex This paper PDB: 6W5V Cryo-EM map of NPC1 in digitonin at pH 8.0 Gong et al., 2016 EMDB: EMD-6640 Cryo-EM map of NPC1-nanodisc This paper EMDB: EMD-21545 Cryo-EM map of NPC1-8.0 This paper EMDB: EMD-21546 (Continued on next page) ll Cell 182, 1–14.e1–e6, July 9, 2020 e1 Please cite this article in press as: Qian et al., Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2, Cell (2020), https://doi.org/10.1016/j.cell.2020.05.020 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM map of NPC1-5.5a This Recombinant:Article Title: Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LAMP-1 Santa Cruz Cat# sc-20011; RRID: AB_626853 Alexa Fluor Plus 594 Donkey anti-Mouse IgG Invitrogen Cat# A32744; RRID: AB_2762826 Bacterial and Virus Strains E. coli Top10 Thermo Fisher Scientific Cat# C404010 E. coli BL21(DE3) Novagen Cat# 69387-1 E. coli DH5a Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins n-Dodecyl-b-D-Maltoside (DDM) Anatrace Cat# D310S glyco-diosgenin (GDN) Anatrace Cat# GDN101 anti-Flag M2 affinity gel MilliporeSigma Cat# A2220 Flag peptide GenScript Cat# RP10586-1 Ni-NTA Agarose QIAGEN Cat# 30250 Dulbecco’s modified eagle’s medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) GIBCO Cat# 10565042 Fetal bovine serum (FBS) GIBCO Cat# 10091148 LB medium Sigma Cat# L3522 Freestyle 293 medium Thermo Fisher Scientific Cat# 12338018 Filipin MilliporeSigma Cat# F9765 Kanamycin MilliporeSigma Cat# K1876 Triton X-100 MilliporeSigma Cat# X-100 Lipofectamine LTX with PLUS Invitrogen Cat# 15338100 ProLong Gold antifade reagent Invitrogen Cat# P10144 DMEM GIBCO Cat# 11995073 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat# 15140163 Foetal Bovine Serum (FBS) Scientifix Cat# FFBS-500 Mycoplasma Removal Agent Bio-Rad Cat# BUF035 Bovine Serum Albumin Bovogen Cat# BSAS-AU Superose 6 Increase 10/300 GL GE Healthcare Cat# 29-0915-96 Superdex 200 Increase 10/300 GL GE Healthcare Cat# 28-9909-44 Source 15Q GE Healthcare Cat# 17094702 Asolectin MilliporeSigma Cat# 11145 Bio-Beads SM-2 Resin Bio-Rad Cat# 1523920 Deposited Data Coordinates of NPC1 in digitonin at pH 8.0 Gong et al., 2016 PDB: 3JD8 Coordinates of NPC1-nanodisc This paper PDB: 6W5R Coordinates of NPC1-8.0 This paper PDB: 6W5S Coordinates of NPC1-5.5a This paper PDB: 6W5T Coordinates of NPC1-5.5b This paper PDB: 6W5U Coordinates of NPC1-NPC2 complex This paper PDB: 6W5V Cryo-EM map of NPC1 in digitonin at pH 8.0 Gong et al., 2016 EMDB: EMD-6640 Cryo-EM map of NPC1-nanodisc This paper EMDB: EMD-21545 Cryo-EM map of NPC1-8.0 This paper EMDB: EMD-21546 (Continued on next page) ll Cell 182, 1–14.e1–e6, July 9, 2020 e1 Please cite this article in press as: Qian et al., Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2, Cell (2020), https://doi.org/10.1016/j.cell.2020.05.020 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM map of NPC1-5.5a This Modification:Article Title: Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LAMP-1 Santa Cruz Cat# sc-20011; RRID: AB_626853 Alexa Fluor Plus 594 Donkey anti-Mouse IgG Invitrogen Cat# A32744; RRID: AB_2762826 Bacterial and Virus Strains E. coli Top10 Thermo Fisher Scientific Cat# C404010 E. coli BL21(DE3) Novagen Cat# 69387-1 E. coli DH5a Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins n-Dodecyl-b-D-Maltoside (DDM) Anatrace Cat# D310S glyco-diosgenin (GDN) Anatrace Cat# GDN101 anti-Flag M2 affinity gel MilliporeSigma Cat# A2220 Flag peptide GenScript Cat# RP10586-1 Ni-NTA Agarose QIAGEN Cat# 30250 Dulbecco’s modified eagle’s medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) GIBCO Cat# 10565042 Fetal bovine serum (FBS) GIBCO Cat# 10091148 LB medium Sigma Cat# L3522 Freestyle 293 medium Thermo Fisher Scientific Cat# 12338018 Filipin MilliporeSigma Cat# F9765 Kanamycin MilliporeSigma Cat# K1876 Triton X-100 MilliporeSigma Cat# X-100 Lipofectamine LTX with PLUS Invitrogen Cat# 15338100 ProLong Gold antifade reagent Invitrogen Cat# P10144 DMEM GIBCO Cat# 11995073 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat# 15140163 Foetal Bovine Serum (FBS) Scientifix Cat# FFBS-500 Mycoplasma Removal Agent Bio-Rad Cat# BUF035 Bovine Serum Albumin Bovogen Cat# BSAS-AU Superose 6 Increase 10/300 GL GE Healthcare Cat# 29-0915-96 Superdex 200 Increase 10/300 GL GE Healthcare Cat# 28-9909-44 Source 15Q GE Healthcare Cat# 17094702 Asolectin MilliporeSigma Cat# 11145 Bio-Beads SM-2 Resin Bio-Rad Cat# 1523920 Deposited Data Coordinates of NPC1 in digitonin at pH 8.0 Gong et al., 2016 PDB: 3JD8 Coordinates of NPC1-nanodisc This paper PDB: 6W5R Coordinates of NPC1-8.0 This paper PDB: 6W5S Coordinates of NPC1-5.5a This paper PDB: 6W5T Coordinates of NPC1-5.5b This paper PDB: 6W5U Coordinates of NPC1-NPC2 complex This paper PDB: 6W5V Cryo-EM map of NPC1 in digitonin at pH 8.0 Gong et al., 2016 EMDB: EMD-6640 Cryo-EM map of NPC1-nanodisc This paper EMDB: EMD-21545 Cryo-EM map of NPC1-8.0 This paper EMDB: EMD-21546 (Continued on next page) ll Cell 182, 1–14.e1–e6, July 9, 2020 e1 Please cite this article in press as: Qian et al., Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2, Cell (2020), https://doi.org/10.1016/j.cell.2020.05.020 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM map of NPC1-5.5a This Plasmid Preparation:Article Title: Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LAMP-1 Santa Cruz Cat# sc-20011; RRID: AB_626853 Alexa Fluor Plus 594 Donkey anti-Mouse IgG Invitrogen Cat# A32744; RRID: AB_2762826 Bacterial and Virus Strains E. coli Top10 Thermo Fisher Scientific Cat# C404010 E. coli BL21(DE3) Novagen Cat# 69387-1 E. coli DH5a Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins n-Dodecyl-b-D-Maltoside (DDM) Anatrace Cat# D310S glyco-diosgenin (GDN) Anatrace Cat# GDN101 anti-Flag M2 affinity gel MilliporeSigma Cat# A2220 Flag peptide GenScript Cat# RP10586-1 Ni-NTA Agarose QIAGEN Cat# 30250 Dulbecco’s modified eagle’s medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) GIBCO Cat# 10565042 Fetal bovine serum (FBS) GIBCO Cat# 10091148 LB medium Sigma Cat# L3522 Freestyle 293 medium Thermo Fisher Scientific Cat# 12338018 Filipin MilliporeSigma Cat# F9765 Kanamycin MilliporeSigma Cat# K1876 Triton X-100 MilliporeSigma Cat# X-100 Lipofectamine LTX with PLUS Invitrogen Cat# 15338100 ProLong Gold antifade reagent Invitrogen Cat# P10144 DMEM GIBCO Cat# 11995073 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat# 15140163 Foetal Bovine Serum (FBS) Scientifix Cat# FFBS-500 Mycoplasma Removal Agent Bio-Rad Cat# BUF035 Bovine Serum Albumin Bovogen Cat# BSAS-AU Superose 6 Increase 10/300 GL GE Healthcare Cat# 29-0915-96 Superdex 200 Increase 10/300 GL GE Healthcare Cat# 28-9909-44 Source 15Q GE Healthcare Cat# 17094702 Asolectin MilliporeSigma Cat# 11145 Bio-Beads SM-2 Resin Bio-Rad Cat# 1523920 Deposited Data Coordinates of NPC1 in digitonin at pH 8.0 Gong et al., 2016 PDB: 3JD8 Coordinates of NPC1-nanodisc This paper PDB: 6W5R Coordinates of NPC1-8.0 This paper PDB: 6W5S Coordinates of NPC1-5.5a This paper PDB: 6W5T Coordinates of NPC1-5.5b This paper PDB: 6W5U Coordinates of NPC1-NPC2 complex This paper PDB: 6W5V Cryo-EM map of NPC1 in digitonin at pH 8.0 Gong et al., 2016 EMDB: EMD-6640 Cryo-EM map of NPC1-nanodisc This paper EMDB: EMD-21545 Cryo-EM map of NPC1-8.0 This paper EMDB: EMD-21546 (Continued on next page) ll Cell 182, 1–14.e1–e6, July 9, 2020 e1 Please cite this article in press as: Qian et al., Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2, Cell (2020), https://doi.org/10.1016/j.cell.2020.05.020 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM map of NPC1-5.5a This Clone Assay:Article Title: Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-LAMP-1 Santa Cruz Cat# sc-20011; RRID: AB_626853 Alexa Fluor Plus 594 Donkey anti-Mouse IgG Invitrogen Cat# A32744; RRID: AB_2762826 Bacterial and Virus Strains E. coli Top10 Thermo Fisher Scientific Cat# C404010 E. coli BL21(DE3) Novagen Cat# 69387-1 E. coli DH5a Invitrogen Cat# 18265017 Chemicals, Peptides, and Recombinant Proteins n-Dodecyl-b-D-Maltoside (DDM) Anatrace Cat# D310S glyco-diosgenin (GDN) Anatrace Cat# GDN101 anti-Flag M2 affinity gel MilliporeSigma Cat# A2220 Flag peptide GenScript Cat# RP10586-1 Ni-NTA Agarose QIAGEN Cat# 30250 Dulbecco’s modified eagle’s medium/Ham’s Nutrient Mixture F-12 (DMEM/F12) GIBCO Cat# 10565042 Fetal bovine serum (FBS) GIBCO Cat# 10091148 LB medium Sigma Cat# L3522 Freestyle 293 medium Thermo Fisher Scientific Cat# 12338018 Filipin MilliporeSigma Cat# F9765 Kanamycin MilliporeSigma Cat# K1876 Triton X-100 MilliporeSigma Cat# X-100 Lipofectamine LTX with PLUS Invitrogen Cat# 15338100 ProLong Gold antifade reagent Invitrogen Cat# P10144 DMEM GIBCO Cat# 11995073 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat# 15140163 Foetal Bovine Serum (FBS) Scientifix Cat# FFBS-500 Mycoplasma Removal Agent Bio-Rad Cat# BUF035 Bovine Serum Albumin Bovogen Cat# BSAS-AU Superose 6 Increase 10/300 GL GE Healthcare Cat# 29-0915-96 Superdex 200 Increase 10/300 GL GE Healthcare Cat# 28-9909-44 Source 15Q GE Healthcare Cat# 17094702 Asolectin MilliporeSigma Cat# 11145 Bio-Beads SM-2 Resin Bio-Rad Cat# 1523920 Deposited Data Coordinates of NPC1 in digitonin at pH 8.0 Gong et al., 2016 PDB: 3JD8 Coordinates of NPC1-nanodisc This paper PDB: 6W5R Coordinates of NPC1-8.0 This paper PDB: 6W5S Coordinates of NPC1-5.5a This paper PDB: 6W5T Coordinates of NPC1-5.5b This paper PDB: 6W5U Coordinates of NPC1-NPC2 complex This paper PDB: 6W5V Cryo-EM map of NPC1 in digitonin at pH 8.0 Gong et al., 2016 EMDB: EMD-6640 Cryo-EM map of NPC1-nanodisc This paper EMDB: EMD-21545 Cryo-EM map of NPC1-8.0 This paper EMDB: EMD-21546 (Continued on next page) ll Cell 182, 1–14.e1–e6, July 9, 2020 e1 Please cite this article in press as: Qian et al., Structural Basis of Low-pH-Dependent Lysosomal Cholesterol Egress by NPC1 and NPC2, Cell (2020), https://doi.org/10.1016/j.cell.2020.05.020 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Cryo-EM map of NPC1-5.5a This |